6/28

6/28

Today we created two more ways to crysrallize lysozome protein.
Free interface diffusion. Capillaries of protein solution are place in vials of salt solution of various concentrations. By adding solute we will create crystals
Vapor diffusion. We put drops of protein solution over a strong concentration resevoir. The drop will slowly evaporate leaving crystals

We also looked at yesterday’s experiment and we have crystals.

We also looked at an old plate(1 year) which was crystallizing an HIV protein,the source was a tobacco plant. Prof Fromme excited because there were good crystals where previously there were none.

Xenon and kryton gasses are used to create a reference point in the crystal structure. Also mercury is used.
To be a good scientist you need
Knowledge in chemistry, biology, physics, patience, sense of adventure, good team work, sense of humor.