The morning was first spent with Raymund Fromme( the husband of Petra Fromme). We were preparing vapor diffusion plates for another protein. It was comforting that I knew what, why and how we were doing the technique.
I then spent an hour with Gabbi. She showed how software is used to analyse the data. First the X-ray diffaction data(1 exposure 8Mb, 500 exposures) is used to create the electron density map. Using more software the electron density map s then used to create an atom structure model. This can be very time consuming. One example took 1 1/2 years as it needed so many iterations.
Finally the atom model can be transfromed into a ribbon model.
Tuesday, July 3, 2007
Subscribe to:
Post Comments (Atom)
Blog Archive
-
▼
2007
(20)
-
▼
July
(13)
- This morning we continued with the gfp extraction....
- Jenniferand I worked with with Di on the GFP extra...
- At this morning's seminar Craig gave an excellent ...
- This morning we took pictures of our lysozyme crys...
- Today we continued our PCR lab. Our cultures had s...
- This morning we spent our time understanding PCR w...
- This morning we spent the morning with Prof Petra ...
- We spent the morning working on x-ray diffraction....
- Here are two photos of philocene protein crystals....
- This morning, Raymund Fromme, led us through the p...
- The morning was first spent with Raymund Fromme( t...
- Here are some vapor diffusion crystals of lysozyme
- Today, I spent the first hour with Ptra Fromme. Sh...
-
▼
July
(13)
No comments:
Post a Comment