This morning we spent the morning with Prof Petra Fromme preparing reaction vessels to create crystals of PS1. PS1 crystallisation uses dialysis to reduce the salt concentration to create crystals.
There were lots of different stages. We had to find the exact molarity of the protein. We did this by finding the molarity of the chlorophyll which we did by using a spectroanalyser.
We had to make dialysis tubes. The salt solution will pass through a membrane to a high salt conc leaving behind protein in a higher salt conc.