Today we continued our PCR lab. Our cultures had spent an hour in the PCR machine and had hopefully multiplied. Now we had to use a gel and electric voltage to see if that was true. We had created the gel yesterday. A sample of each PCR run with a loading dye was put into a small well at the top of the gell. We had 5 samples plus a standard. A volatge of 100V was placed across the gel and left for an hour. To see the DNA we had to add ethidium bromide which binds to the DNA. We then looked at the gel under a UV lamp. DNA had been multplied, the PCR had worked.

We also spent an hour with Professor Wachter discussing green fluorescent proteins(GFP). GFP are an immense area of interest. Nam is creating mutants of GFP to see if the can react in a faster amount, at present 1/2 hour